RT Journal Article
SR Electronic
T1 “ER-Ca<sup>2+</sup> sensor STIM regulates neuropeptides required for development under nutrient restriction in <em>Drosophila</em>”
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 386649
DO 10.1101/386649
A1 Megha
A1 Christian Wegener
A1 Gaiti Hasan
YR 2019
UL http://biorxiv.org/content/early/2019/03/26/386649.abstract
AB Neuroendocrine cells communicate via neuropeptides to regulate behaviour and physiology. This study examines how STIM (Stromal Interacting Molecule), an ER-Ca2+ sensor required for Store-operated Ca2+ entry, regulates neuropeptides required for Drosophila development under nutrient restriction (NR). We find two STIM-regulated peptides, Corazonin and short Neuropeptide F, to be required for NR larvae to complete development. Further, a set of secretory DLP (Dorso lateral peptidergic) neurons which co-express both peptides was identified. Partial loss of dSTIM caused peptide accumulation in the DLPs, and reduced systemic Corazonin signalling. Upon NR, larval development correlated with increased peptide levels in the DLPs, which failed to occur when dSTIM was reduced. Comparison of systemic and cellular phenotypes associated with reduced dSTIM, with other cellular perturbations, along with genetic rescue experiments, suggested that dSTIM primarily compromises neuroendocrine function by interfering with neuropeptide release. Under chronic stimulation, dSTIM also appears to regulate neuropeptide synthesis.