PT - JOURNAL ARTICLE AU - Luke M. Noble AU - Matthew V. Rockman AU - Henrique Teotónio TI - Gene-level quantitative trait mapping in an expanded <em>C. elegans</em> multiparent experimental evolution panel AID - 10.1101/589432 DP - 2019 Jan 01 TA - bioRxiv PG - 589432 4099 - http://biorxiv.org/content/early/2019/03/26/589432.short 4100 - http://biorxiv.org/content/early/2019/03/26/589432.full AB - The Caenorhabditis elegans multiparental experimental evolution (CeMEE) panel is a collection of genome-sequenced, cryopreserved recombinant inbred lines useful for mapping the genetic basis and evolution of quantitative traits. We have expanded the resource with new lines and new populations, and here report updated additive and epistatic mapping simulations and the genetic and haplotypic composition of CeMEE version 2. Additive QTL explaining 3% of trait variance are detected with &gt;80% power, and the median detection interval is around the length of a single gene on the highly recombinant chromosome arms. Although CeMEE populations are derived from a long-term evolution experiment, genetic structure is dominated by variation present in the ancestral population and is not obviously associated with phenotypic differentiation. C. elegans provides exceptional experimental advantages for the study of phenotypic evolution.