%0 Journal Article %A Sweyta Lohani %A Adria K. Martig %A Suzanne M. Underhill %A Alicia DeFrancesco %A Melanie J. Roberts %A Linda Rinaman %A Susan Amara %A Bita Moghaddam %T Burst activation of dopamine neurons produces prolonged post-burst availability of actively released dopamine %D 2017 %R 10.1101/125062 %J bioRxiv %P 125062 %X Both phasic and tonic modes of neurotransmission are implicated in critical functions assigned to dopamine. In learning, for example, sub-second phasic responses of ventral tegmental area (VTA) dopamine neurons to salient events serve as teaching signals, but learning is also interrupted by dopamine antagonists administered minutes after training. Our findings bridge the multiple timescales of dopamine neurotransmission by demonstrating that burst stimulation of VTA dopamine neurons produces a prolonged post-burst increase (> 20 min) of extracellular dopamine in nucleus accumbens and prefrontal cortex. This elevation is not due to spillover from the stimulation surge but depends on impulse flow-mediated dopamine release. We identified Rho-mediated internalization of dopamine transporter as a mechanism responsible for prolonged availability of actively released dopamine. These results demonstrate that phasic and tonic dopamine neurotransmission can be a continuum and may explain why both modes of signaling are critical for motivational and cognitive functions associated with dopamine. %U https://www.biorxiv.org/content/biorxiv/early/2017/04/06/125062.full.pdf