RT Journal Article
SR Electronic
T1 Autonomous combinatorial color barcoding for multiplexing single molecule RNA visualization
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 127373
DO 10.1101/127373
A1 Cheng, Yong-Sheng
A1 Zhuo, Yue
A1 Hartmann, Katharina
A1 Zou, Peng
A1 Bekki, Gözde
A1 Alter, Heike
A1 Liu, Hai-Kun
YR 2017
UL http://biorxiv.org/content/early/2017/04/18/127373.abstract
AB Single molecular fluorescence in situ hybridization (smFISH) detects RNA transcripts with spatial information and digital molecular counting. However, the broad usage of smFISH is still hindered by the complex chemical probe conjugation or microscopy set-up, especially for investigating multiple gene expression. Here we present a multiple fluorophore enzymatic labeling method (termed HuluFISH) for smFISH probes to achieve flexible combinatorial color barcoding in single hybridization step. The multiplex capacity of HuluFISH follows an exponential growth with the increase of the number of fluorophore types. We demonstrate that this method can be used to detect cellular heterogeneity in embryonic mouse brain on single cell level.