PT  - JOURNAL ARTICLE
AU  - Mantas Žurauskas
AU  - Oliver Barnstedt
AU  - Maria Frade-Rodriguez
AU  - Scott Waddell
AU  - Martin J. Booth
TI  - Rapid Sensing of Volumetric Neural Activity through Adaptive Remote Focusing
AID  - 10.1101/125070
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 125070
4099  - http://biorxiv.org/content/early/2017/04/20/125070.short
4100  - http://biorxiv.org/content/early/2017/04/20/125070.full
AB  - The ability to record neural activity in the brain of a living organism at cellular resolution is of great importance for defining the neural circuit mechanisms that direct behavior. Here we present an adaptive two-photon microscope optimized for extraction of neural signals over volumes in intact Drosophila brains, even in the presence of specimen motion. High speed volume imaging was made possible through reduction of spatial resolution while maintaining the light collection efficiency of a high resolution, high numerical aperture microscope. This enabled simultaneous recording of odor-evoked calcium transients in a defined volume of mushroom body Kenyon cell bodies in a live fruit fly.