PT  - JOURNAL ARTICLE
AU  - Pelisch, Federico
AU  - Borja, Laura Bel
AU  - Jaffray, Ellis G.
AU  - Hay, Ronald T.
TI  - Sumoylation regulates central spindle protein dynamics during chromosome segregation in oocytes
AID  - 10.1101/584763
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 584763
4099  - http://biorxiv.org/content/early/2019/03/27/584763.short
4100  - http://biorxiv.org/content/early/2019/03/27/584763.full
AB  - Meiotic spindles in most species lack centrosomes and the mechanisms that underlie faithful chromosome segregation in acentrosomal meiotic spindles are not well understood. In C. elegans oocytes, spindle microtubules exert a poleward force on chromosomes dependent on the microtubule-stabilising protein CLS-2CLASP. The kinase BUB-1Bub1 and CLS-2CLASP localise in the central-spindle and display a dynamic localisation pattern throughout anaphase but the signals regulating their anaphase-specific localisation remains unknown. We have shown that SUMO regulates BUB-1 localisation during metaphase I. Here, we found that SUMO modification of BUB-1Bub1 is regulated by the SUMO E3 ligase GEI-17 and the SUMO protease ULP-1. SUMO is required for BUB-1 localisation in between segregating chromosomes during early anaphase I, and SUMO depletion partially phenocopies BUB-1Bub1 depletion. We also show that CLS-2CLASP is subject to SUMO-mediated regulation. Over-all, we provide evidence for a novel, SUMO-mediated control of protein dynamics during early anaphase I in oocytes.