PT  - JOURNAL ARTICLE
AU  - Linlin Zhang
AU  - Robert D. Reed
TI  - A practical guide to CRISPR/Cas9 genome editing in Lepidoptera
AID  - 10.1101/130344
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 130344
4099  - http://biorxiv.org/content/early/2017/04/24/130344.short
4100  - http://biorxiv.org/content/early/2017/04/24/130344.full
AB  - CRISPR/Cas9 genome editing has revolutionized functional genetic work in many organisms and is having an especially strong impact in emerging model systems. Here we summarize recent advances applying CRISPR/Cas9 methods in Lepidoptera, with a focus on providing practical advice on the entire process of genome editing from experimental design through to genotyping. We also describe successful targeted GFP knock-ins that we have achieved in butterflies. Finally, we provide a complete, detailed protocol for producing targeted long deletions in butterflies.