RT Journal Article
SR Electronic
T1 Quantitative imaging of loop extruders rebuilding interphase genome architecture after mitosis
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2024.05.29.596439
DO 10.1101/2024.05.29.596439
A1 Brunner, Andreas
A1 Morero, Natalia Rosalia
A1 Zhang, Wanlu
A1 Hossain, M. Julius
A1 Lampe, Marko
A1 Pflaumer, Hannah
A1 Halavatyi, Aliaksandr
A1 Peters, Jan-Michael
A1 Beckwith, Kai S.
A1 Ellenberg, Jan
YR 2024
UL http://biorxiv.org/content/early/2024/05/30/2024.05.29.596439.abstract
AB How cells establish the interphase genome organization after mitosis is incompletely understood. Using quantitative and super-resolution microscopy, we show that the transition from a Condensin to a Cohesin-based genome organization occurs dynamically over two hours. While a significant fraction of Condensins remains chromatin-bound until early Gl, Cohesin-STAGl and its boundary factor CTCF are rapidly imported into daughter nuclei in telophase, immediately bind chromosomes as individual complexes and are sufficient to build the first interphase TAD structures. By contrast, the more abundant Cohesin-STAG2 accumulates on chromosomes only gradually later in Gl, is responsible for compaction inside TAD structures and forms paired complexes upon completed nuclear import. 0ur quantitative time-resolved mapping of mitotic and interphase loop extruders in single cells reveals that the nested loop architecture formed by sequential action of two Condensins in mitosis is seamlessly replaced by a less compact, but conceptually similar hierarchically nested loop architecture driven by sequential action of two Cohesins.Competing Interest StatementThe authors have declared no competing interest.