RT Journal Article SR Electronic T1 Moments reconstruction and local dynamic range compression of high order superresolution optical fluctuation imaging JF bioRxiv FD Cold Spring Harbor Laboratory SP 500819 DO 10.1101/500819 A1 Xiyu Yi A1 Sungho Son A1 Ryoko Ando A1 Atsushi Miyawaki A1 Shimon Weiss YR 2019 UL http://biorxiv.org/content/early/2019/03/28/500819.abstract AB Super-resolution Optical Fluctuation Imaging (SOFI) offers a simple and affordable alternative to other super-resolution (SR) imaging techniques. The theoretical resolution enhancement of SOFI scales linearly with the order of cumulants, while the imaging conditions exhibits less photo-toxicity to the living samples as compared to other SR methods. High order SOFI could, therefore, be a method of choice for dynamic live cell imaging. However, due to the cusp-artifacts and dynamic range expansion of pixel intensities, this promise has not been materialized as of yet. Here we investigated and compared high order moments vs. high order cumulants SOFI reconstructions. We demonstrate that even-order moments reconstructions are intrinsically free of cusp artifacts, allowing for a subsequent deconvolution operation to be performed, hence enhancing the resolution even further. High order moments reconstructions performance was examined for various (simulated) conditions and applied to (experimental) imaging of QD labeled microtubules in fixed cells, and actin stress fiber dynamics in live cells.