PT - JOURNAL ARTICLE AU - Rilling, Amelia AU - Power, Geoffrey A. TI - No effect of menstrual cycle phase on eccentric exercise-induced neuromuscular impairments and the magnitude of the repeated bout effect AID - 10.1101/2024.06.10.598231 DP - 2024 Jan 01 TA - bioRxiv PG - 2024.06.10.598231 4099 - http://biorxiv.org/content/early/2024/06/11/2024.06.10.598231.short 4100 - http://biorxiv.org/content/early/2024/06/11/2024.06.10.598231.full AB - Neuromuscular function is impaired following an unaccustomed bout of eccentric exercise, however, through the repeated bout effect (RBE) the muscle is protected from damage following a subsequent bout of eccentric exercise. As a result of unaccustomed eccentric contractions, structural muscle damage occurs in both sexes. However, the inflammatory response may be mitigated in females due to estradiol, thereby attenuating the secondary effects of muscle damage and potentially limiting the magnitude of the RBE. We investigated the relationship between menstrual cycle phase and oral contraceptive use on neuromuscular impairments following the first bout of exercise, and the magnitude of the RBE. Thirteen female participants performed two bouts of 150 maximal eccentric voluntary contractions of the elbow flexors four weeks apart. Normally menstruating females participated during the late follicular phase (day 10-14) of their menstrual cycle, as determined through cycle tracking, when estradiol is near peak, and progesterone is lower. Oral contraceptive users were tested on their placebo pill days (lower estradiol). Neuromuscular function was assessed for Bout 1 before the eccentric protocol and then again 48 hours following, and this was repeated 4 weeks later for Bout 2. Eccentric exercise-induced muscle weakness and soreness did not differ between groups following Bout 1 (p=0.885), and the magnitude of the RBE (p<0.05) was similar between groups (p=0.995). Females in the late follicular phase (classified as high estradiol) and females on combined oral contraceptives (low estradiol) had similar impairments in neuromuscular function following the first bout of eccentric exercise, and a similar RBE.Competing Interest StatementThe authors have declared no competing interest.