RT Journal Article SR Electronic T1 Differential effects of diphenyl diselenide (PhSe)2 on mitochondria-related pathways depending on the cellular energy status in Bovine Vascular Endothelial Cells JF bioRxiv FD Cold Spring Harbor Laboratory SP 2024.06.14.599060 DO 10.1101/2024.06.14.599060 A1 Galant, Letícia Selinger A1 Doblado, Laura A1 Radi, Rafael A1 da Rocha, João Batista Teixeira A1 de Bem, Andreza Fabro A1 Monsalve, Maria YR 2024 UL http://biorxiv.org/content/early/2024/06/14/2024.06.14.599060.abstract AB Cellular energy metabolism varies depending on tissue and cell type, as well as the availability of energy substrates and energy demands. We recently investigated the variations in cellular metabolism and antioxidant responses in primary bovine vascular endothelial cells (BAECs) under different energetic substrate conditions in vitro, specifically glucose or galactose. In this context, pharmacological agents may exert different effects on cells depending on their energy metabolism status. In this study, we aimed to characterize the effects of (PhSe)2, a redox-active molecule known for its prominent cardiovascular effects, on redox-bioenergetic cellular pathways under glycolytic or oxidative conditions in BAECs. Under glucose conditions, (PhSe)2 positively impacted mitochondrial oxidative capacity, as assessed by respirometry, and was associated with changes in mitochondrial cellular dynamics. However, these changes were not observed in cells cultured with galactose. Although (PhSe)2 induced the nuclear translocation of NRF2 in both glucose and galactose media, NRF2 remained in the nuclei of cells cultured in galactose for a longer duration. Additionally, activation of FOXO3a was only detected in galactose media. Notably, (PhSe)2 strongly induced the expression of genes controlling mitochondrial antioxidant capacity and glutathione synthesis and recycling in glucose media, whereas its effects in galactose media were primarily focused on glutathione homeostasis. In conclusion, our findings underscore the critical influence of cellular metabolic status on the antioxidant capacity of redox-active molecules such as (PhSe)2.Competing Interest StatementThe authors have declared no competing interest.