RT Journal Article
SR Electronic
T1 Lineage-specific CDK activity dynamics characterize early mammalian development
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2024.06.14.599098
DO 10.1101/2024.06.14.599098
A1 Saykali, Bechara
A1 Tran, Andy D.
A1 Cornwell, James A.
A1 Caldwell, Matthew A.
A1 Sangsari, Paniz Rezvan
A1 Morgan, Nicole Y.
A1 Kruhlak, Michael J.
A1 Cappell, Steven D.
A1 Ruiz, Sergio
YR 2024
UL http://biorxiv.org/content/early/2024/06/14/2024.06.14.599098.abstract
AB Cyclin-dependent kinases (CDK) are key regulatory enzymes that regulate proliferation dynamics and cell fate in response to extracellular inputs. It remains largely unknown how CDK activity fluctuates and influences cell commitment in vivo during early mammalian development. Here, we generated a transgenic mouse model expressing a CDK kinase translocation reporter (KTR) that enabled quantification of CDK activity in live single cells. By examining pre- and post- implantation mouse embryos at different stages, we observed a progressive decrease in CDK activity in cells from the trophectoderm (TE) prior to implantation. This drop correlated with the establishment of an FGF4-dependent signaling gradient through the embryonic-abembryonic axis. Furthermore, we showed that CDK activity levels do not determine cell fate decisions during pre-implantation development. Finally, we uncovered the existence of conserved regulatory mechanisms in mammals by revealing lineage-specific regulation of CDK activity in TE-like human cells.Competing Interest StatementThe authors have declared no competing interest.