TY - JOUR T1 - Rapid DNA Re-Identification for Cell Line Authentication and Forensics JF - bioRxiv DO - 10.1101/132381 SP - 132381 AU - Sophie Zaaijer AU - Assaf Gordon AU - Daniel Speyer AU - Robert Piccone AU - Yaniv Erlich Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/04/30/132381.abstract N2 - DNA re-identification is used for a broad range of applications, ranging from cell line authentication to crime scene sample identification. However, current re-identification schemes suffer from high latency. Here, we describe a rapid, inexpensive, and portable strategy to re-identify human DNA called MinION sketching. Using data from Oxford Nanopore Technologies’ sequencer, MinION sketching requires only 3min of sequencing and ∼91 random SNPs to identify a sample, enabling near real-time applications of DNA re-identification. This method capitalizes on the vastly growing availability of genomic reference data for individuals and cancer cell lines. Hands-on preparation of the samples can be reduced to <1 hour. This empowers the application of MinION sketching in research settings for routine cell line authentication or in forensics.Software is available at https://github.com/TeamErlich/personal-identification-pipeline ER -