PT - JOURNAL ARTICLE AU - Hu, Shubao AU - Chen, Jinfen AU - Wang, Hui AU - Ji, E AU - Su, Xinxin AU - Zhu, Muyao AU - Xiang, Xiaoyan AU - Gong, Li AU - Zhou, Qiang AU - Xiao, Xin AU - Wu, Ganlin AU - Zha, Hannie TI - The Transcription Factor OsNAC5 Mediates Cadmium Stress Response and Accumulation in Rice AID - 10.1101/2024.04.07.588483 DP - 2024 Jan 01 TA - bioRxiv PG - 2024.04.07.588483 4099 - http://biorxiv.org/content/early/2024/06/20/2024.04.07.588483.short 4100 - http://biorxiv.org/content/early/2024/06/20/2024.04.07.588483.full AB - Cadmium (Cd) is a toxic heavy metal that endangers human health through contaminated rice consumption. Reducing Cd uptake in rice is essential for minimizing grain Cd accumulation. However, the transcriptional regulation of Cd uptake in rice remains largely elusive. This study reveals that the transcription factor OsNAC5 in Oryza sativa positively regulates the Cd transporter gene OsNRAMP1, influencing Cd uptake. OsNAC5 is primarily expressed in roots, localized to the nucleus, and upregulated by Cd-induced H2O2. Knocking out OsNAC5 reduces Cd levels in both shoots and roots and increases Cd sensitivity. OsNRAMP1 expression, enhanced by Cd stress, depends on OsNAC5, which binds to “CATGTG” motifs in the OsNRAMP1 promoter activating its expression. Loss of OsNAC5 function decreases Cd accumulation in rice grains. Our findings elucidate the transcriptional regulation of Cd stress response in rice and suggest biotechnological approaches to reduce Cd uptake in crops.Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.Competing Interest StatementThe authors have declared no competing interest.