PT  - JOURNAL ARTICLE
AU  - Razvan C. Stan
AU  - Camila P. Bonin
AU  - Rose Porto
AU  - Francisco G. Soriano
AU  - Maristela M. de Camargo
TI  - Negative correlation between the expression of <em>tlr4</em> and <em>grp78</em> is characteristic of sepsis onset and progression
AID  - 10.1101/133264
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 133264
4099  - http://biorxiv.org/content/early/2017/05/02/133264.short
4100  - http://biorxiv.org/content/early/2017/05/02/133264.full
AB  - In sepsis caused by Gram-negative bacteria, modulation of Toll-like receptor 4 (TLR4) activity by modulators such as glucose-regulated protein 78 kDa (GRP78), is believed to shift the equilibrium between pro- and anti-inflammatory downstream signaling cascade. We measured daily mRNA tlr4 and grp78 expression levels in peripheral blood of a cohort of septic patients, upon intensive care admission, and modeled these mRNA values based on a sine damping function. We obtained negative correlations between tlr4 and grp78 mRNA in the survivor group. In contrast, such relation is lost in the deceased patients. Effect sizes were 2.035 (tlr4) and 2.168 (grp78). Measuring the correlation patterns of the expression of these two genes may serve as a robust means to gauge sepsis progression, requiring three points of measurement on the first day of hospitalization. Higher levels of grp78 and lower levels of tlr4 anticipated a positive evolution of sepsis, resulting in patient survival.