PT  - JOURNAL ARTICLE
AU  - Micah P. Stumme-Diers
AU  - Siddhartha Banerjee
AU  - Mohtadin Hashemi
AU  - Zhiqiang Sun
AU  - Yuri L. Lyubchenko
TI  - Nanoscale Dynamics of Centromere Nucleosomes and the Critical Roles of CENP-A
AID  - 10.1101/133520
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 133520
4099  - http://biorxiv.org/content/early/2017/05/03/133520.short
4100  - http://biorxiv.org/content/early/2017/05/03/133520.full
AB  - In the absence of a functioning centromere, chromosome segregation becomes aberrant, leading to an increased rate of aneuploidy. The highly specific recognition of centromeres by kinetochores suggests that specific structural characteristics define this region, however, the structural details and mechanism underlying this recognition remains a matter of intense investigation. To address this, High speed atomic force microscopy was used for direct visualization of the spontaneous dynamics of CENP-A nucleosomes at the sub-second time scale. We report that CENP-A nucleosomes change conformation spontaneously and reversibly, utilizing two major pathways: unwrapping, and looping of the DNA; enabling core transfer between neighboring DNA substrates. Along with these nucleosome dynamics we observed that CENP-A stabilizes the histone core against dissociating to histone subunits, unique from H3 cores which are only capable of such plasticity in the presence of remodeling factors. These findings have implications for the dynamics and integrity of nucleosomes at the centromere.