PT - JOURNAL ARTICLE AU - Horan-Portelance, Liam AU - Iba, Michiyo AU - Acri, Dominic J. AU - Gibbs, J. Raphael AU - Masliah, Eliezer AU - Cookson, Mark R. TI - Single-cell spatial transcriptomics reveals molecular patterns of selective neuronal vulnerability to α-synuclein pathology in a transgenic mouse model of Lewy body disease AID - 10.1101/2024.07.31.606032 DP - 2024 Jan 01 TA - bioRxiv PG - 2024.07.31.606032 4099 - http://biorxiv.org/content/early/2024/08/01/2024.07.31.606032.short 4100 - http://biorxiv.org/content/early/2024/08/01/2024.07.31.606032.full AB - One of the unifying pathological hallmarks of Parkinson’s disease (PD) and dementia with Lewy bodies (DLB) is the presence of misfolded, aggregated, and often phosphorylated forms of the protein α-synuclein in neurons. α-Synuclein pathology appears in select populations of neurons throughout various cortical and subcortical regions, and little is currently known about why some neurons develop pathology while others are spared. Here, we utilized subcellular-resolution imaging-based spatial transcriptomics (IST) in a transgenic mouse model that overexpresses wild-type human α-synuclein (α-syn-tg) to evaluate patterns of selective neuronal vulnerability to α-synuclein pathology. By performing post-IST immunofluorescence for α-synuclein phosphorylated at Ser129 (pSyn), we identified cell types in the cortex and hippocampus that were vulnerable or resistant to developing pSyn pathology. Next, we investigated the transcriptional underpinnings of the observed selective vulnerability using a set of custom probes to detect genes involved in α-synuclein processing and toxicity. We identified expression of the kinase:substrate pair Plk2, which phosphorylates α-synuclein at Ser129, and human SNCA (hSNCA), as underlying the selective vulnerability to pSyn pathology. Finally, we performed differential gene expression analysis, comparing non-transgenic cells to pSyn- and pSyn+ α-syn-tg cells to reveal gene expression changes downstream of hSNCA overexpression and pSyn pathology, which included pSyn-dependent alterations in mitochondrial and endolysosomal genes. This study provides a comprehensive use case of IST, yielding new biological insights into the formation of α-synuclein pathology and its downstream effects in a PD/DLB mouse model.Competing Interest StatementThe authors have declared no competing interest.