RT Journal Article
SR Electronic
T1 Single-cell spatial transcriptomics reveals molecular patterns of selective neuronal vulnerability to α-synuclein pathology in a transgenic mouse model of Lewy body disease
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2024.07.31.606032
DO 10.1101/2024.07.31.606032
A1 Horan-Portelance, Liam
A1 Iba, Michiyo
A1 Acri, Dominic J.
A1 Gibbs, J. Raphael
A1 Masliah, Eliezer
A1 Cookson, Mark R.
YR 2024
UL http://biorxiv.org/content/early/2024/08/01/2024.07.31.606032.abstract
AB One of the unifying pathological hallmarks of Parkinson’s disease (PD) and dementia with Lewy bodies (DLB) is the presence of misfolded, aggregated, and often phosphorylated forms of the protein α-synuclein in neurons. α-Synuclein pathology appears in select populations of neurons throughout various cortical and subcortical regions, and little is currently known about why some neurons develop pathology while others are spared. Here, we utilized subcellular-resolution imaging-based spatial transcriptomics (IST) in a transgenic mouse model that overexpresses wild-type human α-synuclein (α-syn-tg) to evaluate patterns of selective neuronal vulnerability to α-synuclein pathology. By performing post-IST immunofluorescence for α-synuclein phosphorylated at Ser129 (pSyn), we identified cell types in the cortex and hippocampus that were vulnerable or resistant to developing pSyn pathology. Next, we investigated the transcriptional underpinnings of the observed selective vulnerability using a set of custom probes to detect genes involved in α-synuclein processing and toxicity. We identified expression of the kinase:substrate pair Plk2, which phosphorylates α-synuclein at Ser129, and human SNCA (hSNCA), as underlying the selective vulnerability to pSyn pathology. Finally, we performed differential gene expression analysis, comparing non-transgenic cells to pSyn- and pSyn+ α-syn-tg cells to reveal gene expression changes downstream of hSNCA overexpression and pSyn pathology, which included pSyn-dependent alterations in mitochondrial and endolysosomal genes. This study provides a comprehensive use case of IST, yielding new biological insights into the formation of α-synuclein pathology and its downstream effects in a PD/DLB mouse model.Competing Interest StatementThe authors have declared no competing interest.