RT Journal Article SR Electronic T1 Rapid reprogramming and stabilisation of homoeolog expression bias in hexaploid wheat biparental populations JF bioRxiv FD Cold Spring Harbor Laboratory SP 2024.08.01.606180 DO 10.1101/2024.08.01.606180 A1 Glombik, Marek A1 Arunkumar, Ramesh A1 Burrows, Samuel A1 Mogg, Sophie Louise A1 Wang, Xiaoming A1 Borrill, Philippa YR 2024 UL http://biorxiv.org/content/early/2024/08/07/2024.08.01.606180.abstract AB Differences in the relative level of expression of homoeologs, known as homoeolog expression bias (HEB), are widely observed in allopolyploids. While the evolution of homoeolog expression bias through hybridisation has been characterised, on shorter timescales the extent to which homoeolog expression bias is preserved or altered between generations remains elusive.Here we use biparental mapping populations of hexaploid wheat (Triticum aestivum) with a common Paragon parent to explore the inheritance of homoeolog expression bias in the F5 generation.We found that homoeolog expression bias is inherited for 26-27% of triads in both populations. Most triads (∼70%) conserved a similar homoeolog expression bias pattern as one or both parents. Inherited patterns were largely driven by changes in the expression of one homoeolog, allowing homoeolog expression bias in subsequent generations to match parental expression. Novel patterns of homoeolog expression bias occurred more frequently in the biparental population from a landrace x elite cross, than in the population with two elite parents.These results demonstrate that there is significant reprogramming and stabilisation of homoeolog expression bias within a small number of generations that differs significantly based on the parental lines used in the crossing.Competing Interest StatementThe authors have declared no competing interest.The data that supports the findings of this study are available in public repositories. Raw RNA-seq data can be obtained through BioProject ID PRJNA1128551 on the NCBI Sequence Read Archive. Scripts and intermediate data tables necessary to run them and create plots for figures are available on GitHub (https://github.com/Borrill-Lab/Inheritance-of-HEB-in-wheat) or figshare (https://figshare.com/projects/Reprogramming_and_stabilisation_of_homoeolog_expression_bias_in_hexaploid_wheat_biparental_populations/214495) depending on their size.