RT Journal Article
SR Electronic
T1 Boosting the localization precision of dSTORM by biocompatible metal-dielectric coated glass coverslips
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 136739
DO 10.1101/136739
A1 Hannah S. Heil
A1 Benjamin Schreiber
A1 Monika Emmerling
A1 Sven Hoefling
A1 Martin Kamp
A1 Markus Sauer
A1 Katrin G. Heinze
YR 2017
UL http://biorxiv.org/content/early/2017/05/11/136739.abstract
AB Super-resolution techniques such as direct Stochastic Optical Reconstruction Microscopy (dSTORM) have become versatile and well-established tools for biological imaging over the last century. Here, we theoretically and experimentally show that clever combination of different fluorescence modalities allows further improvements. We found that the interaction of fluorophores with plasmonic surfaces boost super-resolution performance in dSTORM approaches as it allows for tailoring the excitation and emission properties. The strength of the approach is that no further specialized microscope setup is required as the described enhancement solely rely on metal-dielectric coated glass coverslips that are straightforward to fabricate. Such biocompatible plasmonic nanolayers enhance the signal-to-noise ratio of dSTORM, and thus sharpens the localization precision by a factor of two.