RT Journal Article
SR Electronic
T1 Molecular profiles, sources and lineage restrictions of stem cells in an annelid regeneration model
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2024.07.09.602635
DO 10.1101/2024.07.09.602635
A1 Stockinger, Alexander W.
A1 Adelmann, Leonie
A1 Fahrenberger, Martin
A1 Ruta, Christine
A1 Özpolat, B. Duygu
A1 Milivojev, Nadja
A1 Balavoine, Guillaume
A1 Raible, Florian
YR 2024
UL http://biorxiv.org/content/early/2024/08/23/2024.07.09.602635.abstract
AB Regeneration of missing body parts can be observed in diverse animal phyla, but it remains unclear to which extent these capacities rely on shared or divergent principles. Research into this question requires detailed knowledge about the involved molecular and cellular principles in suitable reference models. By combining single-cell RNA sequencing and mosaic transgenesis in the marine annelid Platynereis dumerilii, we map cellular profiles and lineage restrictions during posterior regeneration. Our data reveal cell-type specific injury responses, re-expression of positional identity factors, and the re-emergence of stem cell signatures in multiple cell populations. Epidermis and mesodermal coelomic tissue produce distinct putative posterior stem cells (PSCs) in the emerging blastema. A novel mosaic transgenesis strategy reveals both developmental compartments and lineage restrictions during regenerative growth. Our work supports the notion that posterior regeneration involves dedifferentiation, and reveals molecular and mechanistic parallels between annelid and vertebrate regeneration.Competing Interest StatementThe authors have declared no competing interest.