RT Journal Article
SR Electronic
T1 RNase reverses segment sequence in the anterior of a beetle egg (<em>Callosobruchus maculatus</em>, Coleoptera)
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 059162
DO 10.1101/059162
A1 Jitse M. van der Meer
YR 2017
UL http://biorxiv.org/content/early/2017/05/13/059162.abstract
AB The genetic regulation of anterior-posterior segment pattern development has been elucidated in detail for Drosophila, but it is not canonical for insects. A surprising diversity of regulatory mechanisms is being uncovered not only between insect Orders, but also within the Order of the Diptera. This raises the question whether the same diversity of regulatory mechanisms exists within other insect Orders. This paper draws attention to the promise of the pea beetle Callosobruchus maculatus for elucidating the evolution of pattern regulation mechanisms in Coleoptera and other insect Orders. Introduction of RNase in eggs of Callosobruchus replaces anterior segments with posterior segments oriented in mirror image symmetry to the original posterior segments (double abdomens). Reversal is specific for RNase activity, for treatment of the anterior egg pole and for cytoplasmic RNA. Yield depends on developmental stage, enzyme concentration and temperature. A maximum of 30% of treated eggs reversed segment sequence after puncture in 10.0 μg/ml RNase S reconstituted from S-protein and S-peptide at 30 °C. This result sets the stage for an analysis of the genetic regulation of segment pattern formation in the long germ embryo of the Coleopteran Callosobruchus and for comparison with the short germ embryo of the Coleopteran Tribolium.