RT Journal Article
SR Electronic
T1 The condensin complex is a mechanochemical motor that translocates along DNA
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 137711
DO 10.1101/137711
A1 Tsuyoshi Terekawa
A1 Shveta Bisht
A1 Jorine M. Eeftens
A1 Cees Dekker
A1 Christian H. Haering
A1 Eric C. Greene
YR 2017
UL http://biorxiv.org/content/early/2017/05/13/137711.abstract
AB One Sentence Summary Single-molecule imaging reveals that eukaryotic condensin is a highly processive DNA-translocating motor complex.Abstract Condensin plays crucial roles in chromosome organization and compaction, but the mechanistic basis for its functions remains obscure. Here, we use single-molecule imaging to demonstrate that Saccharomyces cerevisiae condensin is a molecular motor capable of ATP hydrolysis-dependent translocation along double-stranded DNA. Condensin’s translocation activity is rapid and highly processive, with individual complexes traveling an average distance of ≥10 kilobases at a velocity of ∼60 base pairs per second. Our results suggest that condensin may take steps comparable in length to its ∼50-nanometer coiled-coil subunits, suggestive of a translocation mechanism that is distinct from any reported DNA motor protein. The finding that condensin is a mechanochemical motor has important implications for understanding the mechanisms of chromosome organization and condensation.