RT Journal Article
SR Electronic
T1 Method for determining of cytotoxicity based on the release of fluorescent proteins
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2024.08.31.610658
DO 10.1101/2024.08.31.610658
A1 Lifanov, Dmitry
A1 Zorigt, Dulamsuren
A1 Shabalina, Evgenya
A1 Khalil, Abdullah
A1 Gorbunov, Konstantin
A1 Petersen, Elena
YR 2024
UL http://biorxiv.org/content/early/2024/09/02/2024.08.31.610658.abstract
AB This paper describes a method for determining the cytotoxicity of chemical compounds based on the detection of fluorescent proteins - in this case, green fluorescent protein (GFP) and red fluorescent protein (RFP), which are released into the medium from dead cells. This method is similar in principle to the lactate dehydrogenase test (LDH test), but it does not require a reaction with a chromogenic substrate. This method also makes it possible to independently determine the viability of different lines when used in cocultures. Experiments were preformed on a classical monolayer, spheroids and 3D cultures in alginate hydrogel. Capecitabine was used as a model cytotoxic agent. We included liver cells (Huh7) in a coculture model and determined changes in the cytotoxicity levels of capecitabine against NCI-H1299 cells. The experimental part also found that there were differences in sensitivity to capecitabine depending on the type of 3D cultures used.Competing Interest StatementThe authors have declared no competing interest.