RT Journal Article
SR Electronic
T1 Proteomic Investigation of Neurotrophic <em>trans</em>-Banglene Reveals Potential Link to Iron Homeostasis
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2024.09.04.611284
DO 10.1101/2024.09.04.611284
A1 Gunawardana, Piyumi B. Wijesiri
A1 Gohil, Khyati
A1 Moon, Kyung-Mee
A1 Foster, Leonard J.
A1 Williams, Florence J.
YR 2024
UL http://biorxiv.org/content/early/2024/09/04/2024.09.04.611284.abstract
AB In an effort to gain insight into cellular systems impacted by neurotrophic trans-banglene (t-BG), global proteomic profiling and Western blot analyses were employed. Expression level changes in response to t-BG treatment were compared to those observed with nerve growth factor (NGF), a natural neurotrophic protein and functional analog to t-BG. Findings from these studies did not point to direct interception of NGF/TrkA signaling by t-BG. Instead, significant alterations in iron-binding and iron-regulating proteins were observed. Intracellular iron measurements by FerroOrange indicate lower ferrous (Fe2+) iron levels in t-BG treated cells but not in NGF treated cells. These results highlight a potential connection between iron regulation and neurotrophic activity.Competing Interest StatementThe authors have declared no competing interest.