RT Journal Article
SR Electronic
T1 Sweet science: Exploring the impact of fructose and glucose on brown adipocyte differentiation using optical diffraction tomography
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2024.09.04.611269
DO 10.1101/2024.09.04.611269
A1 Anantha, Pooja
A1 Wu, Xiangdong
A1 Elsaid, Salaheldeen
A1 Raj, Piyush
A1 Barman, Ishan
A1 Tee, Sui Seng
YR 2024
UL http://biorxiv.org/content/early/2024/09/04/2024.09.04.611269.abstract
AB The thermogenic capacity of brown adipose tissue (BAT) has garnered much attention for its potential to regulate systemic energy balance. BAT depot size and function need to be tightly to prevent loss of metabolic homeostasis due to energy dissipation via non-shivering thermogenesis. While adipocyte-intrinsic mechanisms controlling thermogenesis are critical, an increasing appreciation for the role of the BAT microenvironment is emerging. For example, changes in circulating hexoses due to dietary intake have shown to impact BAT function. Here, we show that murine BAT preadipocytes metabolism is impacted when fructose is used as the sole carbon source. Similarly, differentiation medium containing only fructose yield mature adipocytes with fewer lipid droplets, with a concomitant decrease in adipogenic genes. These deficiencies are also observed in human BAT preadipocytes, where cutting-edge optical imaging modalities show a decrease in total cell mass and lipid mass in fructose-only medium. Taken together, the metabolic microenvironment significantly impacts BAT growth and function, with implications for the role of diets potentially mitigating the efficacy of BAT-targeted therapies.Competing Interest StatementThe authors have declared no competing interest.