PT  - JOURNAL ARTICLE
AU  - Bernhard Schmierer
AU  - Sandeep K. Botla
AU  - Jilin Zhang
AU  - Mikko Turunen
AU  - Teemu Kivioja
AU  - Jussi Taipale
TI  - CRISPR/Cas9 screening using unique molecular identifiers
AID  - 10.1101/114355
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 114355
4099  - http://biorxiv.org/content/early/2017/05/15/114355.short
4100  - http://biorxiv.org/content/early/2017/05/15/114355.full
AB  - Loss of function screening by CRISPR/Cas9 gene knockout with pooled, lentiviral guide libraries is a widely applicable method for systematic identification of genes contributing to diverse cellular phenotypes. Here, random sequence labels (RSLs) are incorporated into the guide library, which act as unique molecular identifiers (UMIs) to allow massively parallel lineage tracing and lineage dropout screening. RSLs greatly improve the reproducibility of results by increasing both the precision and the accuracy of screens. They reduce the number of cells and sequencing reads needed to reach a set statistical power, or allow a more robust screen using the same number of cells.