RT Journal Article
SR Electronic
T1 RNase H1 and H2 are differentially regulated to eliminate RNA-DNA hybrids
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 593152
DO 10.1101/593152
A1 Arianna Lockhart
A1 Vanessa Borges Pires
A1 Fabio Bento
A1 Vanessa Kellner
A1 Sarah Luke-Glaser
A1 Brian Luke
YR 2019
UL http://biorxiv.org/content/early/2019/03/30/593152.abstract
AB RNA-DNA hybrids are tightly regulated to ensure genome integrity. The RNase H enzymes, RNase H1 and H2, contribute to chromosomal stability through the removal of RNA-DNA hybrids. Loss of RNase H2 function is implicated in human diseases of the nervous system and cancer. To better understand RNA-DNA hybrid dynamics, we have focused on elucidating the regulation of the RNase H enzymes themselves. Using yeast as a model system, we demonstrate that RNase H1 and H2 are controlled in different manners. RNase H2 is regulated in a strict cell cycle dependent manner, both in terms of its R-loop removal, and ribonucleotide excision repair functions. RNase H1, however, can function independent of cell cycle stage to remove R-loops, but appears to become activated in response to high R-loop loads. These results provide us with a more complete understanding of how and when RNA-DNA hybrids are acted upon by the RNase H enzymes.