RT Journal Article
SR Electronic
T1 High-Definition Electronic Genome Maps from Single Molecule Data
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 139840
DO 10.1101/139840
A1 Oliver, John S.
A1 Catalano, Anthony
A1 Davis, Jennifer R.
A1 Grinberg, Boris S.
A1 Hutchins, Timothy E.
A1 Kaiser, Michael D.
A1 Nurnberg, Steve
A1 Sage, Jay M.
A1 Seward, Leah
A1 Simelgor, Gregory
A1 Weiner, Nathan K.
A1 Bready, Barrett
YR 2017
UL http://biorxiv.org/content/early/2017/05/18/139840.abstract
AB With the advent of routine short-read genome sequencing has come a growing recognition of the importance of long-range, structural information in applications ranging from sequence assembly to the detection of structural variation. Here we describe the Nabsys solid-state detector capable of detecting tags on single molecules of DNA 100s of kilobases in length as they translocate through the detector at a velocity greater than 1 megabase pair per second. Sequence-specific tags are detected with a high signal-to-noise ratio. The physical distance between tags is determined after accounting for viscous drag-induced intramolecular velocity fluctuations. The accurate measurement of the physical distance between tags on each molecule and the ability of the detector to resolve distances between tags of less than 300 base-pairs enables the construction of high-density genome maps.