RT Journal Article SR Electronic T1 Validating the genus Pocheina (Acrasidae, Heterolobosea, Excavata) leads to the recognition of three major lineages within Acrasidae JF bioRxiv FD Cold Spring Harbor Laboratory SP 2024.10.05.616810 DO 10.1101/2024.10.05.616810 A1 Tice, Alexander K. A1 Regis, Kevin A1 Shutt, Timothy E. A1 Spiegel, Frederick W. A1 Brown, Matthew W. A1 Silberman, Jeffery D. YR 2024 UL http://biorxiv.org/content/early/2024/10/06/2024.10.05.616810.abstract AB Pocheina and Acrasis are two genera of heterolobosean sorocarpic amoebae within Acrasidae that have historically been considered close relatives. The two genera were differentiated based on their differing fruiting body morphologies. The validity of this taxonomic distinction was challenged when a SSU rRNA phylogenetic study placed an isolate morphologically identified as ‘Pocheina’ rosea within a clade of Acrasis rosea isolates. The authors speculated that pocheinoid fruiting body morphology might be the result of aberrant A. rosea fruiting body development, which if true, would nullify this taxonomic distinction between genera. To clarify Acrasidae systematics, we analyzed SSU rRNA and ITS region sequences from multiple isolates of Pocheina, Acrasis, and Allovahlkampfia generated by PCR and transcriptomics. We demonstrate that the initial SSU sequence attributed to ‘P. rosea’ originated from an A. rosea DNA contamination in its amplification reaction. Our analyses, based on morphology, SSU and 5.8S rRNA genes phylogenies, as well as comparative analyses of ITS1 and ITS2 sequences, resolve Acrasidae into three major lineages; Allovahlkampfia and the strongly supported clades comprising Pocheina and Acrasis. We confirm that the latter two genera can be identified by their fruiting body morphologies.Competing Interest StatementThe authors have declared no competing interest.