TY - JOUR T1 - Identification of a native novel oncolytic immunoglobulin on exfoliated colon epithelial cells: A bispecific heterodimeric chimera of IgA/IgG JF - bioRxiv DO - 10.1101/140673 SP - 140673 AU - George P. Albaugh AU - Sudhir K. Dutta AU - Vasantha Iyengar AU - Samina Shami AU - Althaf Lohani AU - Eduardo Sainz AU - George Kessie AU - Prasanna Nair AU - Sara Lagerholm AU - Alka Kamra AU - J-H Joshua Chen AU - Shilpa Kalavapudi AU - Robert Shores AU - Laila E. Phillips AU - Ram Nair AU - Padmanabhan P Nair Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/05/22/140673.abstract N2 - Understanding the nature of cell surface markers on exfoliated colonic cells is a crucial step in establishing criteria for a normally functioning mucosa. We have found that colonic cells isolated from stool samples (SCSR-010 Fecal Cell Isolation Kit, NonInvasive Technologies, Elkridge, MD), preserved at room temperature for up to one week, with viability of >85% and low levels of apoptosis (8% - 10%) exhibit two distinct cell size subpopulations, in the 2.5μM– 5.0 μM and 5.0μM-8.0μM range. In addition to IgA, about 60% of the cells expressed a novel heterodimeric IgA/IgG immunoglobulin that conferred a broad-spectrum cell mediated cytotoxicity against tumor cells. In a cohort of 58 subjects the exclusive absence of this immunoglobulin in two African-Americans was suggestive of a germline deletion. Serial cultures in stem cell medium retained the expression of this heterodimer. Since a majority of the cystic cells expressed the stem cell markers Lgr5 and Musashi-1 we termed these cells as gastrointestinal progenitor stem cells (GIP-C**). CXCR-4, the cytokine co-receptor for HIV was markedly expressed. These cells also expressed CD20, IgA, IgG, CD45, and COX-2. We assume that they originated from mature columnar epithelium by dedifferentiation. Our observations indicate that we have a robust noninvasive method to study mucosal pathphysiology and a direct method to create a database for applications in regenerative medicine. ER -