TY - JOUR T1 - Spatial organization of the mouse auditory cortex to sound dynamics revealed using automated image segmentation JF - bioRxiv DO - 10.1101/139659 SP - 139659 AU - Ji Liu AU - Matthew R. Whiteway AU - Daniel A. Butts AU - Patrick O. Kanold Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/05/23/139659.abstract N2 - Sound stimuli are characterized by their rich spectral and temporal dynamic properties. Individual neurons in auditory cortex (ACX) encode both spectral and temporal aspects of sounds e.g. sound onset and/or offset. While the different fields of the ACX show gradients of frequency selectivity the large-scale organization of sound dynamics is unknown. We used widefield imaging of GCaMP6s in awake mouse ACX combined with a novel unsupervised image segmentation technique to investigate the spatiotemporal representation of sound onset and offset. Using this technique, we identified known auditory fields but also detected novel ACX areas. Furthermore, we found that ACX areas differed in their responses to tone onset and offset. Multiple areas were preferentially activated by tone offset, and on-response areas were more spatially localized than off-response areas. We also found tonotopy in off-responses. Together our results demonstrate a different spatial distribution of neurons across ACX for processing sound onsets versus offsets. ER -