RT Journal Article
SR Electronic
T1 Specific Virus-Host Genome Interactions Revealed by Tethered Chromosome Conformation Capture
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 142604
DO 10.1101/142604
A1 Haochen Li
A1 Reza Kalhor
A1 Bing Li
A1 Trent Su
A1 Arnold J. Berk
A1 Siavash K. Kurdistani
A1 Frank Alber
A1 Lin Chen
YR 2017
UL http://biorxiv.org/content/early/2017/05/26/142604.abstract
AB Viruses have evolved a variety of mechanisms to interact with host cells for their adaptive benefits, including subverting host immune responses and hijacking host DNA replication/transcription machineries [1–3]. Although interactions between viral and host proteins have been studied extensively, little is known about how the vial genome may interact with the host genome and how such interactions could affect the activities of both the virus and the host cell. Since the three-dimensional organization of a genome can have significant impact on genomic activities such as transcription and replication, we hypothesize that such structure-based regulation of genomic functions also applies to viral genomes depending on their association with host genomic regions and their spatial locations inside the nucleus. Here, we used Tethered Chromosome Conformation Capture (TCC) to investigate viral-host genome interactions between the adenovirus and human lung fibroblast cells. We found viral-host genome interactions were enriched in certain active chromatin regions and chromatin domains marked by H3K27me3. The contacts by viral DNA seems to impact the structure and function of the host genome, leading to remodeling of the fibroblast epigenome. Our study represents the first comprehensive analysis of viral-host interactions at the genome structure level, revealing unexpectedly specific virus-host genome interactions. The non-random nature of such interactions indicates a deliberate but poorly understood mechanism for targeting of host DNA by foreign genomes.