RT Journal Article
SR Electronic
T1 The peach RGF/GLV signalling peptide pCTG134 is involved in a regulatory circuit that sustains auxin and ethylene actions
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 141705
DO 10.1101/141705
A1 Nicola Busatto
A1 Umberto Salvagnin
A1 Francesca Resentini
A1 Silvia Quaresimin
A1 Lorella Navazio
A1 Oriano Marin
A1 Maria Pellegrini
A1 Fabrizio Costa
A1 Dale F. Mierke
A1 Livio Trainotti
YR 2017
UL http://biorxiv.org/content/early/2017/05/26/141705.abstract
AB Peach is a climacteric species whose ripening is regulated by the plant hormone ethylene. A crosstalk mechanism with auxin is necessary to support climacteric ethylene synthesis. The homeostasis control of auxin is regulated also by the activity of peptide hormones (PHs), acting both as short and long distant ligands. In this work, we investigated the role of CTG134, a peach gene encoding a GOLVEN-like PH isolated in mesocarp at the onset of ripening.In peach fruit, CTG134 was expressed during the climacteric transition and its mRNA level was induced by auxin and 1-methylcyclopropene (1-MCP) treatments, whereas it was minimally affected by ethylene. To better elucidate its function, CTG134 was overexpressed in Arabidopsis and tobacco, which showed abnormal root hair growth, similar to wild-type plants treated with a synthetic form of the peptide. Molecular surveys demonstrated an impaired hormonal crosstalk, resulting in a re-modulated expression of a set of genes involved in both ethylene and auxin domains. In addition, the promoter of pCTG134 fused with GUS reporter highlighted gene activity in plant organs in which the auxin-ethylene interplay is known to occur. These data support the role of pCTG134 as mediator in an auxin-ethylene regulatory circuit.Highlight The role of the peach RGF/GLV peptide during root hair formation in Arabidopsis and tobacco supports its involvement in a cross-hormonal auxin-ethylene regulatory circuit.