PT  - JOURNAL ARTICLE
AU  - Linlin Dong
AU  - Jiang Xu
AU  - Lianjuan Zhang
AU  - Guangfei Wei
AU  - He Su
AU  - Juan Yang
AU  - Jun Qian
AU  - Ran Xu
AU  - Baosheng Liao
AU  - Liang Shen
AU  - Mingli Wu
AU  - Ruiyang Cheng
AU  - Shilin Chen
TI  - Inoculation of biocontrol bacteria alleviated <em>Panax ginseng</em> replanting problem
AID  - 10.1101/143412
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 143412
4099  - http://biorxiv.org/content/early/2017/05/29/143412.short
4100  - http://biorxiv.org/content/early/2017/05/29/143412.full
AB  - Replanting problem is a common and serious issue hindering the continuous cultivation of Panax plants. Changes in soil microbial community driven by plant species of different ages and developmental stages are speculated to cause this problem. Inoculation of microbial antagonists is proposed to alleviate replanting issues efficiently.High-throughput sequencing revealed that bacterial diversity evidently decreased, and fungal diversity markedly increased in soils of adult ginseng plants in the root growth stage. Relatively few beneficial microbe agents, such as Luteolibacter, Cytophagaceae, Luteibacter, Sphingomonas, Sphingomonadaceae, and Zygomycota, were observed. On the contrary, the relative abundance of harmful microorganism agents, namely, Brevundimonas, Enterobacteriaceae, Pandoraea, Cantharellales, Dendryphion, Fusarium, and Chytridiomycota, increased with pant age. Furthermore, Bacillus subtilis 50-1 was isolated and served as microbial antagonists against pathogenic Fusarium oxysporum of ginseng root-rot, and its biocontrol efficacy was 67.8% using a dual culture assay. The ginseng death rate and relative abundance of Fusarium decreased by 63.3% and 46.1%, respectively, after inoculation with 50-1 in replanting soils. Data revealed that changes in the diversity and composition of rhizospheric microbial communities driven by ginseng of different ages and developmental stages could cause microecological degradation. Biocontrol using microbial antagonists was an effective method for alleviating the replanting problem.Highlight Changes in rhizospheric microbial communities driven by ginseng plants 13 of different ages and developmental stages could cause microecological degradation. 14 Biocontrol using microbial antagonists effectively alleviated the replanting problem.