RT Journal Article
SR Electronic
T1 Lipid transfer from plants to arbuscular mycorrhiza fungi
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 143883
DO 10.1101/143883
A1 Andreas Keymer
A1 Priya Pimprikar
A1 Vera Wewer
A1 Claudia Huber
A1 Mathias Brands
A1 Simone L. Bucerius
A1 Pierre-Marc Delaux
A1 Verena Klingl
A1 Edda von Röpenack-Lahaye
A1 Trevor L. Wang
A1 Wolfgang Eisenreich
A1 Peter Dörmann
A1 Martin Parniske
A1 Caroline Gutjahr
YR 2017
UL http://biorxiv.org/content/early/2017/05/30/143883.abstract
AB Arbuscular mycorrhiza (AM) symbioses contribute to global carbon cycles as plant hosts divert up to 20% of photosynthate to the obligate biotrophic fungi. Previous studies suggested carbohydrates as the only form of carbon transferred to the fungi. However, de novo fatty acid (FA) synthesis has not been observed in AM fungi in absence of the plant. In a forward genetic approach, we identified two Lotus japonicus mutants defective in AM-specific paralogs of lipid biosynthesis genes (KASI and GPAT6). These mutants perturb fungal development and accumulation of emblematic fungal 16:1ω5 FAs. Using isotopolog profiling we demonstrate that 13C patterns of fungal FAs recapitulate those of wild-type hosts, indicating cross-kingdom lipid transfer from plants to fungi. This transfer of labelled FAs was not observed for the AM-specific lipid biosynthesis mutants. Thus, growth and development of beneficial AM fungi is not only fueled by sugars but depends on lipid transfer from plant hosts.