RT Journal Article
SR Electronic
T1 Small-Angle and Quasi-Elastic Neutron Scattering from Polydisperse Oligolamellar Vesicles Containing Glycolipids
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2024.11.26.625379
DO 10.1101/2024.11.26.625379
A1 Bange, Lukas
A1 Rahimzadeh, Amin
A1 Mukhina, Tetiana
A1 von Klitzing, Regine
A1 Hoffmann, Ingo
A1 Schneck, Emanuel
YR 2024
UL http://biorxiv.org/content/early/2024/11/29/2024.11.26.625379.abstract
AB Glycolipids are known to stabilize biomembrane multilayers through preferential sugar-sugar interactions that act as weak transient membrane cross-links. Here, we use small-angle and quasi-elastic neutron scattering on oligolamellar phospholipid vesicles containing defined glycolipid fractions in order to elucidate the influence of glycolipids on membrane mechanics and dynamics. Small-angle neutron scattering (SANS) reveals that the oligolamellar vesicles (OLVs) obtained by extrusion are polydisperse with regard to the number of lamellae, n, which renders the interpretation of the quasi-elastic neutron spin echo (NSE) data non-trivial. To overcome this problem, we propose a method to model the NSE data in a rigorous fashion based on the obtained histograms of n and on their q-dependent intensity-weighted contribution. This procedure yields meaningful values for the bending rigidity of individual lipid membranes and insights into the mechanical coupling between adjacent membrane lamellae, including the effect of the glycolipids.Competing Interest StatementThe authors have declared no competing interest.