@article {Kim143917, author = {Eun Ji Kim and Gregory G. Grant and Anita S. Bowman and Naqi Haider and Harini V. Gudiseva and Venkata Ramana Murthy Chavali}, title = {Complete Transcriptome Profiling of Normal and Age-Related Macular Degeneration Eye Tissues Reveals Changes in Regulation of Non-Coding RNA and Extreme Disregulation of Anti-Sense Transcription}, elocation-id = {143917}, year = {2017}, doi = {10.1101/143917}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Strand specific RNA sequencing of retina and RPE-Choroid-Scleara (RCS) in age-related macular degeneration (AMD) and matched normal controls reveals striking impact on anti-sense transcription and changes in the regulation of non-coding RNA that has not previously been reported. Hundreds of genes, which do not express anti-sense transcripts in normal retina and RCS, demonstrate extreme anti-sense expression in AMD. And conversely anti-sense transcription is completely abrogated in many genes which express a high level of anti-sense transcripts in normal retina and RCS. Several pathways are very highly enriched in the upregulated anti-sense transcripts - in particular the EIF2 signaling pathway. These results call for a deeper investigation into anti-sense and noncoding RNA regulation in AMD and their potential as therapeutic targets.}, URL = {https://www.biorxiv.org/content/early/2017/05/30/143917}, eprint = {https://www.biorxiv.org/content/early/2017/05/30/143917.full.pdf}, journal = {bioRxiv} }