PT  - JOURNAL ARTICLE
AU  - Ashley M. Rasys
AU  - Sungdae Park
AU  - Rebecca E. Ball
AU  - Aaron J. Alcala
AU  - James D. Lauderdale
AU  - Douglas B. Menke
TI  - CRISPR-Cas9 Gene Editing in Lizards Through Microinjection of Unfertilized Oocytes
AID  - 10.1101/591446
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 591446
4099  - http://biorxiv.org/content/early/2019/03/31/591446.short
4100  - http://biorxiv.org/content/early/2019/03/31/591446.full
AB  - CRISPR-Cas9 mediated gene editing has enabled the direct manipulation of gene function in many species. However, the reproductive biology of reptiles presents unique barriers for the use of this technology, and there are currently no reptiles with effective methods for targeted mutagenesis. Here we present a new approach that enables the efficient production of CRISPR-Cas9 induced mutations in Anolis lizards, an important model for studies of reptile evolution and development.