RT Journal Article
SR Electronic
T1 The Human Cytoplasmic Dynein Interactome Reveals Novel Activators of Motility
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 143743
DO 10.1101/143743
A1 Redwine, William B.
A1 DeSantis, Morgan E.
A1 Hollyer, Ian
A1 Htet, Zaw Min
A1 Tran, Phuoc Tien
A1 Swanson, Selene K.
A1 Florens, Laurence
A1 Washburn, Michael P.
A1 Reck-Peterson, Samara L.
YR 2017
UL http://biorxiv.org/content/early/2017/05/30/143743.abstract
AB In human cells, cytoplasmic dynein-1 is essential for long-distance transport of many cargos, including organelles, RNAs, proteins, and viruses, towards microtubule minus ends. To understand how a single motor achieves cargo specificity, we identified the human dynein interactome or “transportome” by attaching a promiscuous biotin ligase (“BioID”) to seven components of the dynein machinery, including a subunit of the essential cofactor dynactin. This method reported spatial information about the large cytosolic dynein/dynactin complex in living cells. To achieve maximal motile activity and to bind its cargos, human dynein/dynactin requires “activators”, of which only five have been described. We developed methods to identify new activators in our BioID data, and discovered that ninein and ninein-like are a new family of dynein activators. Analysis of the protein interactomes for six activators, including ninein and ninein-like, suggests that each dynein activator has multiple cargos.