TY - JOUR T1 - Protein profiling in cancer cell lines and tumor tissue using reverse phase protein arrays JF - bioRxiv DO - 10.1101/144535 SP - 144535 AU - Xiaohong Jing AU - Weiqing Wang AU - Nicholas P. Gauthier AU - Poorvi Kaushik AU - Alex Root AU - Richard R. Stein AU - Anil Korkut AU - Chris Sander Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/05/31/144535.abstract N2 - Reverse phase protein array (RPPA) technology is an antibody-based high-throughput assay for protein profiling of biological specimens that allows for many measurements with very small amounts of cell lysate. Here, we report the sensitivity, reproducibility, and accuracy of a particular RPPA platform called Zeptosens. We customized the RPPA protocol for our in-house setup, and measured more than 80 total protein and phospho-protein levels in various cancer samples, including cell lines, organoids, tumor chunks, core needle biopsies, and laser-capture microdissected tissue samples. We discuss pros and cons of the RPPA platform, and describe results from profiling 15 cancer cell line cells using RPPA. ER -