PT - JOURNAL ARTICLE AU - German Tischler TI - Haplotype and Repeat Separation in Long Reads AID - 10.1101/145474 DP - 2017 Jan 01 TA - bioRxiv PG - 145474 4099 - http://biorxiv.org/content/early/2017/06/02/145474.short 4100 - http://biorxiv.org/content/early/2017/06/02/145474.full AB - Resolving the correct structure and succession of highly similar sequence stretches is one of the main open problems in genome assembly. For non haploid genomes this includes determining the sequences of the different haplotypes. For all but the smallest genomes it also involves separating different repeat instances. In this paper we discuss methods for resolving such problems in third generation long reads by classifying alignments between long reads according to whether they represent true or false read overlaps. The main problem in this context is the high error rate found in such reads, which greatly exceeds the amount of difference between the similar regions we want to separate. Our methods can separate read classes stemming from regions with as little as 1% difference.