RT Journal Article
SR Electronic
T1 Deciphering Molecular Cascades in a Novel Acclimatization Strategy for Rapid Ascent to High Altitude
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 145342
DO 10.1101/145342
A1 Subhojit Paul
A1 Anamika Gangwar
A1 Kalpana Bhargava
A1 Yasmin Ahmad
YR 2017
UL http://biorxiv.org/content/early/2017/06/02/145342.abstract
AB The repercussions of hypobaric hypoxia are dependent upon two factors-time and intensity of exposure. The effects of intensity i.e. variation of altitude are yet unknown although it is a significant factor in terms of acclimatization protocols. In this study we present the effects of acute (24 h) exposure to high (10,000 ft), very high (15,000 ft) and extreme altitude (25,000 ft) zones on lung and plasma using semi-quantitative redox specific transcripts and quantitative proteo-bioinformatics workflow in conjunction with redox stress assays. Our findings indicate that very high altitude exposure elicits systemic redox homeostatic processes due to failure of lung redox homeostasis without causing mortality. We also document a rapid acclimatization protocol causing a shift from 0 to 100% survival at 25,000 ft in male SD rats upon rapid induction. Finally we posit the various processes involved and the plasma proteins that can be used to ascertain the acclimatization status of an individual.