RT Journal Article
SR Electronic
T1 A novel phyllosphere resident <em>Protomyces</em> species that interacts with the <em>Arabidopsis</em> immune system
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 594028
DO 10.1101/594028
A1 Kai Wang
A1 Timo Sipilä
A1 Sitaram Rajaraman
A1 Omid Safronov
A1 Pia Laine
A1 Agate Auzane
A1 Alfredo Mari
A1 Petri Auvinen
A1 Lars Paulin
A1 Eric Kemen
A1 Jarkko Salojärvi
A1 Kirk Overmyer
YR 2019
UL http://biorxiv.org/content/early/2019/03/31/594028.abstract
AB We describe the genome contents of six Protomyces species that are pathogenic within the typical host range of the genus and a novel Protomyces strain (SC29) that was previously isolated from the phylloplane of wild Arabidopsis thaliana (Arabidopsis), an atypical host. Genome-wide phylogenetic analysis defined SC29 as a distinct Protomyces species. Analysis of gene family expansions, gene retention, and gene loss patterns among the Protomyces suggests that SC29 has recently undergone a host jump and change in lifestyle. Genomic changes in SC29 were consistent with a phylloplane lifestyle. SC29 did not cause disease on Arabidopsis, but could persist in its phylloplane, while the closely related P. inouyei does not. SC29 treated Arabidopsis exhibited enhanced immunity against Botrytis cinerea infection, associated with activation of MAPK3/6, camalexin, and SA-signalling pathways. We conclude that SC29 is a novel Protomyces species adapted to life in the Arabidopsis phylloplane.