PT - JOURNAL ARTICLE AU - Li, Qin AU - Bai, Huarong AU - Yu, Xueliang AU - Liu, Qiang AU - Hu, Rongkuan TI - Immunogenicity of Rabies Virus G-Protein mRNA Formulated with Muscle Targeting Lipid Nanoparticles in Mice AID - 10.1101/2025.01.06.631441 DP - 2025 Jan 01 TA - bioRxiv PG - 2025.01.06.631441 4099 - http://biorxiv.org/content/early/2025/01/07/2025.01.06.631441.short 4100 - http://biorxiv.org/content/early/2025/01/07/2025.01.06.631441.full AB - Rabies is a preventable zoonotic disease caused by the rabies virus (RABV) with a high mortality rate. Most vaccines on the market or under development have issues such as a low single-dose neutralization titer, complex processes, and high costs. During the COVID-19 pandemic, the successful development of mRNA vaccines has opened up a new avenue for preventive vaccines. As a new technology, mRNA has higher scalability. In this study, we designed an mRNA encoding the RV-G protein, encapsulated by our own muscle targeting lipid nanoparticles (LNP), and evaluated the expression of the RV-G protein in vitro, its immunogenicity, and its protection against virus infection in vivo. The results showed that RV-G mRNA was significantly expressed in vitro. High Virus-IgG binding titers and Virus-neutralizing antibody titers (VNT) were induced by immunization with RV-G mRNA-LNP. Additionally, our results show that the RV-G mRNA vaccine is better than commercially available vaccines in mice.Competing Interest StatementThe authors have declared no competing interest.