RT Journal Article SR Electronic T1 Immunogenicity of Rabies Virus G-Protein mRNA Formulated with Muscle Targeting Lipid Nanoparticles in Mice JF bioRxiv FD Cold Spring Harbor Laboratory SP 2025.01.06.631441 DO 10.1101/2025.01.06.631441 A1 Li, Qin A1 Bai, Huarong A1 Yu, Xueliang A1 Liu, Qiang A1 Hu, Rongkuan YR 2025 UL http://biorxiv.org/content/early/2025/01/07/2025.01.06.631441.abstract AB Rabies is a preventable zoonotic disease caused by the rabies virus (RABV) with a high mortality rate. Most vaccines on the market or under development have issues such as a low single-dose neutralization titer, complex processes, and high costs. During the COVID-19 pandemic, the successful development of mRNA vaccines has opened up a new avenue for preventive vaccines. As a new technology, mRNA has higher scalability. In this study, we designed an mRNA encoding the RV-G protein, encapsulated by our own muscle targeting lipid nanoparticles (LNP), and evaluated the expression of the RV-G protein in vitro, its immunogenicity, and its protection against virus infection in vivo. The results showed that RV-G mRNA was significantly expressed in vitro. High Virus-IgG binding titers and Virus-neutralizing antibody titers (VNT) were induced by immunization with RV-G mRNA-LNP. Additionally, our results show that the RV-G mRNA vaccine is better than commercially available vaccines in mice.Competing Interest StatementThe authors have declared no competing interest.