PT  - JOURNAL ARTICLE
AU  - DT Severson
AU  - RP Owen
AU  - MJ White
AU  - X Lu
AU  - B Schuster-Böckler
TI  - BEARscc determines robustness of single-cell clusters using simulated technical replicates
AID  - 10.1101/118919
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 118919
4099  - http://biorxiv.org/content/early/2017/06/05/118919.short
4100  - http://biorxiv.org/content/early/2017/06/05/118919.full
AB  - Technical variance is a major confounding factor in single-cell RNA sequencing, not least because it is not possible to replicate measurements on the same cell. We present BEARscc, a tool that uses RNA spike-in controls to simulate experiment-specific technical replicates. We demonstrate that the tool improves the unsupervised classification of cells and facilitates the biological interpretation of single-cell RNA-seq experiments.