RT Journal Article
SR Electronic
T1 Distinct neuronal activity patterns induce different gene expression programs
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 146282
DO 10.1101/146282
A1 Kelsey M. Tyssowski
A1 Ramendra N. Saha
A1 Nicholas R. DeStefino
A1 Jin-Hyung Cho
A1 Richard D. Jones
A1 Sarah M. Chang
A1 Palmyra Romeo
A1 Mary K. Wurzelmann
A1 James M. Ward
A1 Serena M. Dudek
A1 Jesse M. Gray
YR 2017
UL http://biorxiv.org/content/early/2017/06/05/146282.abstract
AB Brief and sustained neuronal activity patterns can have opposite effects on synaptic strength that both require activity-regulated gene (ARG) expression. However, whether distinct patterns of activity induce different sets of ARGs is unknown. In genome-scale experiments, we reveal that a neuron’s activity-pattern history can be predicted from the ARGs it expresses. Surprisingly, brief activity selectively induces a small subset of the ARG program that that corresponds precisely to the first of three temporal waves of genes induced by sustained activity. These first-wave genes are distinguished by an open chromatin state, proximity to rapidly activated enhancers, and a requirement for MAPK/ERK signaling for their induction. MAPK/ERK mediates rapid RNA polymerase recruitment to promoters, as well as enhancer RNA induction but not histone acetylation at enhancers. Thus, the same mechanisms that establish the multi-wave temporal structure of ARG induction also enable different sets of genes to be induced by distinct activity patterns.