RT Journal Article SR Electronic T1 Impairment of zebrafish reproduction upon exposure to melengestrol acetate JF bioRxiv FD Cold Spring Harbor Laboratory SP 146506 DO 10.1101/146506 A1 Xiong, Kewen A1 Zhong, Chunyun A1 Wang, Xin YR 2017 UL http://biorxiv.org/content/early/2017/06/06/146506.abstract AB Synthetic progestins contamination is common in the aquatic ecosystem, which may lead to serious health problem on aquatic animals. Melengestrol acetate (MGA) has been detected in the aquatic environment; however, its potential effects on fish reproduction are largely unclear. Here, we aimed to investigate the endocrine disruption and impact of MGA on zebrafish reproduction. Six-month old reproductive zebrafish were exposed to four nominal concentrations of MGA (1,10, 100 and 200 ng/L) for 15 days. Treatment with MGA reduced the egg production with a significant decrease at 200 ng/L. The circulating concentrations of estradiol and testosterone in female zebrafish or 11-keto testosterone in male zebrafish were significantly diminished compared to the non-exposed control fish. The early embryonic development or hatching rates were unaffected during the MGA exposure. Our results indicated that MGA was a potent endocrine disruptor in fish and the fish reproduction could be impaired even during a short-term exposure to MGA.