PT  - JOURNAL ARTICLE
AU  - Razvan C. Stan
AU  - Camila P. Bonin
AU  - Rose Porto
AU  - Francisco G. Soriano
AU  - Maristela M. de Camargo
TI  - Higher levels of <em>grp78</em> and lower levels of <em>tlr4</em> anticipate a positive evolution of sepsis and patient survival
AID  - 10.1101/133264
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 133264
4099  - http://biorxiv.org/content/early/2017/06/06/133264.short
4100  - http://biorxiv.org/content/early/2017/06/06/133264.full
AB  - In sepsis caused by Gram-negative bacteria, modulation of Toll-like receptor 4 (TLR4) activity by modulators such as glucose-regulated protein 78 kDa (GRP78), is believed to shift the equilibrium between pro- and anti-inflammatory downstream signaling cascade. We measured daily mRNA tlr4 and grp78 expression levels in peripheral blood of a cohort of septic patients, upon intensive care admission, and modeled these mRNA values based on a sine damping function. We obtained negative correlations between tlr4 and grp78 mRNA in the survivor group. In contrast, such relation is lost in the deceased patients. Loss of homeostasis predicted by our model within the initial 5 days of hospitalization was confirmed by death of those patients up to 28 days later. Measuring the correlation patterns of the expression of these two genes serves as a robust means to gauge sepsis progression, requiring only three points of measurement on the first day of hospitalization.